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The GDE is a set of programs for multiple sequence alignment and analysis. The GDE currently runs on SUN SparcStations using OpenWindows 2.0 or MIT X11R4. The programs use an expandable user interface which allows the addition of external analysis functions without any rewriting of code. Analysis functions can be written in any language ('C',Fortran, Pascal Basic, shell scripts), and can be seamlessly merged into the systems menu/ dialog box interface. The system supports several data types, nucleic and amino acid sequences, text, and masking sequence, and three forms of color highlighting. The system can handle enormous alignments limited only by the amount of virtual memory on your workstation. Sequences can be edited under several levels of protection, as well as by aligned groups. The system has several external analysis functions included for such things as automated alignment, searching, homology recognition, and phylogenetic analysis. The programs are not in the public domain, but are and will continue to be available for free. Distribution includes full source code, and binaries along with a users manual. Steven Smith Director of Computation Harvard Genome Lab WWW: